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1132 amino acids sequence  (Bioss)


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    Structured Review

    Bioss 1132 amino acids sequence
    1132 Amino Acids Sequence, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1132+amino+acids+sequence/TERT+Polyclonal+Antibody/pmc08324818-80-38-45
    Average 94 stars, based on 14 article reviews
    1132 amino acids sequence - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Membrane:

    Article Title: Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice
    Article Snippet: The isolated and pooled follicles at each stage were immersed in the lysis buffer [composed of 1% sodium dodecyl sulfate (SDS), 1.0 mM sodium ortho-vanadate, and 10 mM Tris; pH 7.4], incubated on ice for 10 min and then centrifuged at 15,000 rpm, at + 4 °C for 10 min. After boiling the samples in 4 × Laemmli buffer (Sigma-Aldrich, St. Louis, MO, USA), they were run on a 10% SDS–polyacrylamide gel electrophoresis (SDS-PAGE) to separate the proteins based on their molecular weight. .. These proteins were transferred to a PVDF membrane (Roche, Indianapolis, IN, USA) and incubated with anti-TERT antibody [specific to the amino acids sequence ranging 650–700 (locating inside the reverse transcriptase domain of human TERT protein) out of total 1132 amino acids sequence; catalog number bs-0233R, Bioss, Massachusetts, USA; diluted 1:2000 in 5% (w/v) non-fat dry milk in 1 × TBST] overnight at + 4 °C on a rotary shaker. ..

    Incubation:

    Article Title: Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice
    Article Snippet: The isolated and pooled follicles at each stage were immersed in the lysis buffer [composed of 1% sodium dodecyl sulfate (SDS), 1.0 mM sodium ortho-vanadate, and 10 mM Tris; pH 7.4], incubated on ice for 10 min and then centrifuged at 15,000 rpm, at + 4 °C for 10 min. After boiling the samples in 4 × Laemmli buffer (Sigma-Aldrich, St. Louis, MO, USA), they were run on a 10% SDS–polyacrylamide gel electrophoresis (SDS-PAGE) to separate the proteins based on their molecular weight. .. These proteins were transferred to a PVDF membrane (Roche, Indianapolis, IN, USA) and incubated with anti-TERT antibody [specific to the amino acids sequence ranging 650–700 (locating inside the reverse transcriptase domain of human TERT protein) out of total 1132 amino acids sequence; catalog number bs-0233R, Bioss, Massachusetts, USA; diluted 1:2000 in 5% (w/v) non-fat dry milk in 1 × TBST] overnight at + 4 °C on a rotary shaker. ..

    Sequencing:

    Article Title: Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice
    Article Snippet: The isolated and pooled follicles at each stage were immersed in the lysis buffer [composed of 1% sodium dodecyl sulfate (SDS), 1.0 mM sodium ortho-vanadate, and 10 mM Tris; pH 7.4], incubated on ice for 10 min and then centrifuged at 15,000 rpm, at + 4 °C for 10 min. After boiling the samples in 4 × Laemmli buffer (Sigma-Aldrich, St. Louis, MO, USA), they were run on a 10% SDS–polyacrylamide gel electrophoresis (SDS-PAGE) to separate the proteins based on their molecular weight. .. These proteins were transferred to a PVDF membrane (Roche, Indianapolis, IN, USA) and incubated with anti-TERT antibody [specific to the amino acids sequence ranging 650–700 (locating inside the reverse transcriptase domain of human TERT protein) out of total 1132 amino acids sequence; catalog number bs-0233R, Bioss, Massachusetts, USA; diluted 1:2000 in 5% (w/v) non-fat dry milk in 1 × TBST] overnight at + 4 °C on a rotary shaker. ..

    Reverse Transcription:

    Article Title: Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice
    Article Snippet: The isolated and pooled follicles at each stage were immersed in the lysis buffer [composed of 1% sodium dodecyl sulfate (SDS), 1.0 mM sodium ortho-vanadate, and 10 mM Tris; pH 7.4], incubated on ice for 10 min and then centrifuged at 15,000 rpm, at + 4 °C for 10 min. After boiling the samples in 4 × Laemmli buffer (Sigma-Aldrich, St. Louis, MO, USA), they were run on a 10% SDS–polyacrylamide gel electrophoresis (SDS-PAGE) to separate the proteins based on their molecular weight. .. These proteins were transferred to a PVDF membrane (Roche, Indianapolis, IN, USA) and incubated with anti-TERT antibody [specific to the amino acids sequence ranging 650–700 (locating inside the reverse transcriptase domain of human TERT protein) out of total 1132 amino acids sequence; catalog number bs-0233R, Bioss, Massachusetts, USA; diluted 1:2000 in 5% (w/v) non-fat dry milk in 1 × TBST] overnight at + 4 °C on a rotary shaker. ..



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    Bioss 1132 amino acids sequence
    1132 Amino Acids Sequence, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1132+amino+acids+sequence/TERT+Polyclonal+Antibody/pmc08324818-80-38-45
    Average 94 stars, based on 1 article reviews
    1132 amino acids sequence - by Bioz Stars, 2026-10
    94/100 stars
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